《植物生理学报》 2015, 51(5): 763-770

两种变异类型沙地柏冬季叶色变化及其与抗氧化系统的关系

曹盼盼, 贾桂霞^*

北京林业大学园林学院, 花卉种质创新与分子育种北京市重点实验室, 国家花卉工程技术研究中心, 城乡生态环境北京实验室, 北京100083

通信作者：贾桂霞；E-mail: gxjia@bjfu.edu.cn；Tel: 13521615634

摘　要：

为探讨冬季叶色变化原因及其与抗氧化的关系, 本文以黄绿色和灰绿色变异类型的沙地柏为研究对象, 观测叶色及色素含量、受胁迫程度和抗氧化系统等生理指标的变化。结果表明, 在自然越冬过程中, 黄绿色的叶色由黄绿色转为橙红色, 灰绿色的由绿色变为灰绿或灰色; 与此同时, 黄绿色叶片的花色素苷(Acy)显著升高, 叶绿素(Chl)及类胡萝卜素(Car)含量变化不明显, 灰绿色叶片的Chl含量显著降低, Car及Acy含量显著升高。在反应植物受胁迫程度的指标中, 过氧化氢(H₂O₂)、相对电导率(EL)及丙二醛(MDA)的含量在入冬后逐渐升高, 次年1或2月份达到最高, 之后下降; 最大光化学效率(F_v/F_m)则表现为相反的变化趋势, 次年1月份最低。在抗氧化系统中, 超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸(AsA)及黄绿色变异类型中的还原型谷胱甘肽(GSH)呈现与H₂O₂相似的变化趋势, 而灰绿色中的GSH含量则呈现高-低-高-低的变化趋势。相关性分析结果表明, 两种变异类型的沙地柏色素和抗氧化系统各组分之间存在着共调节, 以避免或降低冬季造成的氧化伤害, 且灰绿色变异类型的比黄绿色的更能建立较完善的保护系统。

关键词：沙地柏; 变异类型; 叶色; 抗氧化系统

收稿：2014-12-17   修定：2015-03-15

资助：国家林业局科技成果推广计划([2010]27)。

Changes of Leaf Color and Relationship with Antioxidant System in Two Types of Sabina vulgaris in Winter

CAO Pan-Pan, JIA Gui-Xia^*

Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China

Corresponding author: JIA Gui-Xia; E-mail: gxjia@bjfu.edu.cn; Tel: 13521615634

Abstract:

To study the phenomenon of leaf color changes and their relationships with antioxidant system of both yellow-green and gray-green types of Sabina vulgaris in winter, the leaf color, pigments content, physiological indexs of antioxidant systems were observed and measured in this study. The results demonstrated that, in the natural course of the winter, the color of S. vulgaris with yellow-green leaves changed from bright yellow-green to dark orange-red, while the gray-green types changed from green to gray-green cedar or gray. Meanwhile, for the yellow-green types, the content of anthocyanin (Acy) increased significantly, and chlorophyll (Chl) and carotenoid (Car) changed unconspicuously. However, for gray-green types, the content of Chl reduced significantly, and Car and Acy increased significantly. For the physiological indexs reflecting the degree of stress, the content of hydrogen peroxide (H₂O₂), relative conductivity leakage (EL) and malondialdehyde (MDA) increased at first and reached to the peak in January or February next year; while the maximum photochemical efficiency (F_v/F_m) showed the opposite trends and decreased to the bottom in January. For the antioxidant system, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbic acid (AsA) and reduced glutathione (GSH) in yellow-green types presented the same trends as H₂O₂; while GSH content in gray-green types presented the trends of high-low-high-low. The correspondence analyses naturally came to the conclusion that there was a common regulation mechanism which was composed of pigment and antioxidant system to avoid or reduce the oxidative damage in winter. Furthermore, the gray-green types of S. vulgaris could establish a more perfect protection system than the yellow-green types.

Key words: Sabina vulgaris; variation type; leaf color; antioxidant system